Session: 06-05 Implants and Biomedicine

Paper Number: 167434

167434 - Co-Axial and Transverse Axial Electrospraying of Polymer and Biomolecules to Produce Nanocapsules for Targeted Therapy 

We report a novel fabrication process of co- axial electrospraying to produce nanoparticles/nanocapsules of cancer drugs, ligands, inhibitors with biocompatible and biodegradable polymer shells in nanoscale for targeted therapy Moreover, a novel transverse electrospraying nozzle is designed to integrate with the coaxial spinneret to electrospray peptides, ligands or antibody, fluorescent dye on the outer layer of the nanocapsules while processing the nanocapsules. We processed injectable nanonized encapsulated breast cancer drugs for targeted and image guided cancer site specific controlled delivery. Our innovation lies in creating multilayered (Drugs, Inhibitors Polymer, and Peptides or ligands) hydrodynamic cone-jet pulsation of co-axial compound jets with external force (Constant/switching voltage). The co-axial jet pulsation mode in relation to the solution viscosity, solution miscibility and incompatibility, solution conductivities and spray voltage duration lead to a transformative and scalable nanonization and nano-encapsulation of cancer drugs for targeted therapy. In this process, core drugs solution and sheath polymer solution are forced by an electrostatic potential to eject out through different but co-axial capillary channels, resulting in a core shell structure. One advantage in using such a technique is an effective protection of denatured biological agents and the potential to wrap all substances in the core regardless of drug-polymer interactions. Hence, drugs, proteins, growth factors, and even genes can be incorporated into nanostructures Electrospraying of multiple cancer drugs (paclitaxel and GW2580) as core and polycaprolactam (PCL) as the shell has been developed. We have successfully made nanocapsules containing paclitaxel to target breast cancer cells and GW2580, a colony- stimulating factor 1 receptor (CSF1R) inhibitor to target CSF1R+ myeloid cells in the tumor microenvironments (TME). The UV–vis drug release test for 14 days shows a prolonged and sustained release pattern of both the drugs. In vitro and in vivo results showed the effects of nanocapsules containing multiple drugs in controlling the growth of tumor cells and increased survival of the animal bearing breast cancers. Also, In-vivo biodistribution of PCL and CD206 M2 macrophage targeting peptide (TAMS-1) nanoparticles after intravenous injection in the tumor mice model showed uptake to the tumors. On the other hand, MDSC peptide did not show any uptake to the site of tumors. Most activity is shown in the intestine indicating excretion of the agents through the hepato-biliary system.The rationale behind this size range is to utilize the EPR effects of a hypervascular tumor. Structural abnormalities in activated neovascularization result in a leaky vasculature and a poor lymphatic drainage system, which causes a differential interstitial pressure. The 10-100 nm nanoparticles utilize this difference in pressure to preferentially accumulate and be retained in tumors unlike the free drugs or small molecules that rapidly undergo renal filtration.

Presenting Author: Khaleda Akter Georgia Southern University

Presenting Author Biography: Ms. Akter is one of the brightest graduate researchers who started her doctoral studies in the Department of Mechanical Engineering. Her PhD research work is a collaborative work between the ME Department and Georgia Cancer Center, Augusta University .Her investigation involved to process injectable nanonized encapsulated breast cancer drugs (Nanomedicine) for targeted and image guided cancer site specific controlled delivery. Ms. Akter received her B.S. and M.S. in Physics from the University of Dhaka, Bangladesh. She completed her second M.S. in Mechanical Engineering from the University of South Carolina, USA in 2021. In her M.S. research, she developed a novel methodology for anisotropic guided waves in isotropic metaplates, demonstrating strong analytical and problem-solving skills. Her research work was published in the high impact ‘International Journal of Solids and Structures’. She also presented her research at peer-reviewed conferences (Proc. SPIE PC12048, Health Monitoring of Structural and Biological Systems XVI)